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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
01/10/2019 |
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Actualizado : |
01/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASTILLO, A.; CARRO, B. L.; DALLA RIZZA, M.; REYNO, R. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; B. LÓPEZ CARRO, Flow Cytometry and Cell Sorting Core, Institute of Biological Research Clemente Estable, Montevideo, Uruguay.; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Use of in vitro methods to induce autotetraploids in the native forage legume Trifolium polymorphum. |
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Complemento del título : |
Conference Paper. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Acta Horticulturae, 15 November 2018, N° 1224, p. 73-80. |
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Serie : |
(Acta Horticulturae; 1224) |
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ISBN : |
978-94-62612-20-4 |
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ISSN : |
0567-7572 (print) / 2406-6168 (electronic) |
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DOI : |
10.17660/ActaHortic.2018.1224.11 |
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Idioma : |
Inglés |
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Notas : |
In: Acta Hortic. 1224. VII International Symposium on Production and Establishment of Micropropagated Plants. Editors: R. Paiva, M.V. Reis, D.P.C. Silva. |
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Contenido : |
ABSTRACT.
The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum
belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical
environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to
Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor
that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two
antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture
medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of
colchicine reached to 13% of chromosome duplication, and 16% when colchicine (0.015%) was added in the culture medium. The numbers of chloroplasts in stomatal
guard cells was used as a quick assessment of the ploidy level and then confirmed by flow cytometry.
@ISHS, International Society for Horticultural Science. MenosABSTRACT.
The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum
belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical
environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to
Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor
that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two
antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture
medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of
colchicine r... Presentar Todo |
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Palabras claves : |
BIOMASS; COLCHICINE; ORYZALIN; POLYPLOIDY. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02755naa a2200265 a 4500
001 1060254
005 2019-10-01
008 2018 bl uuuu u00u1 u #d
020 $a978-94-62612-20-4
022 $a0567-7572 (print) / 2406-6168 (electronic)
024 7 $a10.17660/ActaHortic.2018.1224.11$2DOI
100 1 $aCASTILLO, A.
245 $aUse of in vitro methods to induce autotetraploids in the native forage legume Trifolium polymorphum.$h[electronic resource]
260 $c2018
490 $a(Acta Horticulturae; 1224)
500 $aIn: Acta Hortic. 1224. VII International Symposium on Production and Establishment of Micropropagated Plants. Editors: R. Paiva, M.V. Reis, D.P.C. Silva.
520 $aABSTRACT. The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of colchicine reached to 13% of chromosome duplication, and 16% when colchicine (0.015%) was added in the culture medium. The numbers of chloroplasts in stomatal guard cells was used as a quick assessment of the ploidy level and then confirmed by flow cytometry. @ISHS, International Society for Horticultural Science.
653 $aBIOMASS
653 $aCOLCHICINE
653 $aORYZALIN
653 $aPOLYPLOIDY
700 1 $aCARRO, B. L.
700 1 $aDALLA RIZZA, M.
700 1 $aREYNO, R.
773 $tActa Horticulturae, 15 November 2018, N° 1224, p. 73-80.
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
07/06/2022 |
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Actualizado : |
30/11/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MACHADO, M.; CASTRO, M.B.; WILSON, T. M.; GONÇALVES , A. A. B.; PORTIANSKY, E.; RIET-CORREA, F.; BARROS, S. S. |
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Afiliación : |
MIZAEL MACHADO DA COSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MÁRCIO B. CASTRO, University of Brasilia, Brasília, Brazil; TAIS M. WILSON, University of Brasilia, Brasília, Brazil; ALEXANDRA A. B. GONÇALVES, University of Brasilia, Brasília, Brazil; ENRIQUE L. PORTIANSKY, National University of La Plata, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Federal University of Bahia, Salvador, Brazil; SEVERO S. BARROS, Federal University of Santa Maria, Rio Grande do Sul, Brazil. |
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Título : |
Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Veterinary Pathology, 2022, Volume 59, Issue 5, pages 814-823. doi: https://doi.org/10.1177/03009858221098430 |
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ISSN : |
0300-9858 |
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DOI : |
10.1177/03009858221098430 |
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Idioma : |
Inglés |
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Notas : |
Article history: Article first published online May 19, 2022; Published online September 2022. -- Corresponding Author: Franklin Riet-Correa, Postgraduate Program in Animal Science in the Tropics, School of Veterinary Medicine and Animal Science, Federal University of Bahia, Salvador, Bahia 40170-110, Brazil. Email: franklinrietcorrea@gmail.com -- This work was funded by National Institute of Agricultural Research (INIA) of Uruguay (Project CL 44), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil, (Finance Code 001) and National University of La Plata (Project V270). A postgraduate scholarship was provided to Mizael Machado by INIA, Uruguay. |
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Contenido : |
ABSTRACT - Vascular mineralization is a hallmark of enzootic calcinosis. Histopathological, ultrastructural, and immunohistochemical investigations were performed on the external carotid arteries of seven sheep naturally poisoned by Nierembergia veitchii. Histologically, moderate to marked hyperplasia of the tunica intima was observed without mineralization. The tunica media exhibited mild to severe mineralization and osteochondroid metaplasia. Sheep with enzootic calcinosis showed arterial overexpression of osteopontin and tissue-nonspecific alkaline phosphatase and immunolabeling for osteonectin and osteocalcin in both intima and media layers of the tested arteries. The main ultrastructural finding in the tunica media was a marked phenotypic change of vascular smooth muscle cells from a contractile phenotype (VSMC-C) into a synthetic phenotype (VSMC-S). In the tunica media, VSMC-S produced matrix and extracellular vesicles, forming mineralizable granules associated with arterial mineralization. VSMC-S were also present in the tunica intima, but matrix and extracellular vesicles and mineralization were not observed. The absence of matrix and extracellular vesicles in the intimal hyperplasia, even in the presence of noncollagenous bone proteins, tissue-nonspecific alkaline phosphatase, and vitamin D receptors, reinforces the hypothesis that the presence of matrix and extracellular vesicles are crucial for the development of vascular mineralization in enzootic calcinosis. It is proposed that the two different VSMC-S phenotypes in calcinosis are due to the expression of at least two genetically different types of these cells induced by the action of 1,25(OH)2D3. © The Author(s) 2022. MenosABSTRACT - Vascular mineralization is a hallmark of enzootic calcinosis. Histopathological, ultrastructural, and immunohistochemical investigations were performed on the external carotid arteries of seven sheep naturally poisoned by Nierembergia veitchii. Histologically, moderate to marked hyperplasia of the tunica intima was observed without mineralization. The tunica media exhibited mild to severe mineralization and osteochondroid metaplasia. Sheep with enzootic calcinosis showed arterial overexpression of osteopontin and tissue-nonspecific alkaline phosphatase and immunolabeling for osteonectin and osteocalcin in both intima and media layers of the tested arteries. The main ultrastructural finding in the tunica media was a marked phenotypic change of vascular smooth muscle cells from a contractile phenotype (VSMC-C) into a synthetic phenotype (VSMC-S). In the tunica media, VSMC-S produced matrix and extracellular vesicles, forming mineralizable granules associated with arterial mineralization. VSMC-S were also present in the tunica intima, but matrix and extracellular vesicles and mineralization were not observed. The absence of matrix and extracellular vesicles in the intimal hyperplasia, even in the presence of noncollagenous bone proteins, tissue-nonspecific alkaline phosphatase, and vitamin D receptors, reinforces the hypothesis that the presence of matrix and extracellular vesicles are crucial for the development of vascular mineralization in enzootic calcinosis. It ... Presentar Todo |
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Palabras claves : |
Arterial mineralization; Arteries; Enzootic calcinosis; Exosomes; Pathogenesis; PLATAFORMA DE SALUD ANIMAL; Toxicity; Vascular smooth muscle cells. |
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Asunto categoría : |
L50 Fisiología y bioquímica animal |
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Marc : |
LEADER 03391nam a2200313 a 4500
001 1063233
005 2022-11-30
008 2022 bl uuuu u01u1 u #d
022 $a0300-9858
024 7 $a10.1177/03009858221098430$2DOI
100 1 $aMACHADO, M.
245 $aPoisoning by Nierembergia veitchii$bEffects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis.$h[electronic resource]
260 $aVeterinary Pathology, 2022, Volume 59, Issue 5, pages 814-823. doi: https://doi.org/10.1177/03009858221098430$c1177
500 $aArticle history: Article first published online May 19, 2022; Published online September 2022. -- Corresponding Author: Franklin Riet-Correa, Postgraduate Program in Animal Science in the Tropics, School of Veterinary Medicine and Animal Science, Federal University of Bahia, Salvador, Bahia 40170-110, Brazil. Email: franklinrietcorrea@gmail.com -- This work was funded by National Institute of Agricultural Research (INIA) of Uruguay (Project CL 44), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil, (Finance Code 001) and National University of La Plata (Project V270). A postgraduate scholarship was provided to Mizael Machado by INIA, Uruguay.
520 $aABSTRACT - Vascular mineralization is a hallmark of enzootic calcinosis. Histopathological, ultrastructural, and immunohistochemical investigations were performed on the external carotid arteries of seven sheep naturally poisoned by Nierembergia veitchii. Histologically, moderate to marked hyperplasia of the tunica intima was observed without mineralization. The tunica media exhibited mild to severe mineralization and osteochondroid metaplasia. Sheep with enzootic calcinosis showed arterial overexpression of osteopontin and tissue-nonspecific alkaline phosphatase and immunolabeling for osteonectin and osteocalcin in both intima and media layers of the tested arteries. The main ultrastructural finding in the tunica media was a marked phenotypic change of vascular smooth muscle cells from a contractile phenotype (VSMC-C) into a synthetic phenotype (VSMC-S). In the tunica media, VSMC-S produced matrix and extracellular vesicles, forming mineralizable granules associated with arterial mineralization. VSMC-S were also present in the tunica intima, but matrix and extracellular vesicles and mineralization were not observed. The absence of matrix and extracellular vesicles in the intimal hyperplasia, even in the presence of noncollagenous bone proteins, tissue-nonspecific alkaline phosphatase, and vitamin D receptors, reinforces the hypothesis that the presence of matrix and extracellular vesicles are crucial for the development of vascular mineralization in enzootic calcinosis. It is proposed that the two different VSMC-S phenotypes in calcinosis are due to the expression of at least two genetically different types of these cells induced by the action of 1,25(OH)2D3. © The Author(s) 2022.
653 $aArterial mineralization
653 $aArteries
653 $aEnzootic calcinosis
653 $aExosomes
653 $aPathogenesis
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aToxicity
653 $aVascular smooth muscle cells
700 1 $aCASTRO, M.B.
700 1 $aWILSON, T. M.
700 1 $aGONÇALVES , A. A. B.
700 1 $aPORTIANSKY, E.
700 1 $aRIET-CORREA, F.
700 1 $aBARROS, S. S.
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